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CRISPR-Cas: A Laboratory Manual by Jennifer Doudna

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  • CRISPR-Cas: A Laboratory Manual
  • Jennifer Doudna
  • Page: 450
  • Format: pdf, ePub, mobi, fb2
  • ISBN: 9781621821311
  • Publisher: Cold Spring Harbor Laboratory Press

Download CRISPR-Cas: A Laboratory Manual




Online audio books free download CRISPR-Cas: A Laboratory Manual iBook 9781621821311 by Jennifer Doudna

CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular biologists modify DNA sequences and modulate gene expression in cells and organisms. This laboratory manual presents step-by-step protocols for applying this cutting-edge technology to any system of interest. Contributors describe approaches for designing guide RNAs that precisely target the gene of interest, delivering CRISPR-Cas reagents into cells (e.g., using AAV vectors), and selecting cells that harbor the desired changes. Strategies for scaling and multiplexing these procedures are also described, as are methods for optimizing CRISPR-Cas systems in common species and cell lines, from yeast to human.

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: ISBN: 9781621821311 : CRISPR-Cas: A Laboratory Manual
CRISPR-Cas-based techniques are revolutionizing the way geneticists and molecular This laboratory manual presents step-by-step protocols for applying this 
Cold Spring Harbor Lab Press Developmental Biology
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.
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Cover image for Crispr/Cas · More Info · Crispr/Cas: A Laboratory Manual | Paperback Jennifer Doudna | Prashant Mali Cold Spring Harbor Laboratory Press
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Crispr/Cas: A Laboratory Manual von University Jennifer Doudna
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While laboratory strains of Escherichia coli contain a functional CRISPR/Cas CRISPR cassettes are often associated with cas genes (Jansen et al., 2002b; Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual.
Cold Spring Harbor Lab Press Books
CRISPR-Cas: A Laboratory Manual Edited By Jennifer Doudna, HHMI/University of California Berkeley; Prashant Mali, University of California San Diego.

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